Concomitant purification of prostatic carcinoma tumor markers from human seminal fluid under nondenaturing conditions.
نویسندگان
چکیده
We report a protocol for concomitant purification to homogeneity of both prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2] and prostate-specific antigen, from human seminal fluid. The method requires only two chromatographic steps: passage through an Affigel-Blue column and gel filtration HPLC. This is a fast, efficient procedure for purification of these two important tumor markers, which are specific for prostatic cancer.
منابع مشابه
One-step, high-yield purification of human prostatic acid phosphatase from seminal fluid by gel-filtration HPLC under nondenaturing conditions.
This is a fast, efficient method for purification to homogeneity of human prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2], from seminal fluid. Use of a "high-pressure" liquid-chromatographic gel-filtration column permits high-yield recovery of the purified enzyme with most of its enzymatic and immunological activity retained.
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عنوان ژورنال:
- Clinical chemistry
دوره 34 12 شماره
صفحات -
تاریخ انتشار 1988